Spinning-disk UltraView VOX

Inverted microscope, equipped with a Yokagawa CSUX1 spinning- disk, and two cameras to do dual-color simultaneous imaging.

It allows the fast acquisition of optically sectioned slices at very high speed, and is compatible with live specimen imaging as it limits phototoxicity.

It is equipped with a FRAP module that can do sequential FRAP stimulation, based on a quick scanner.

Two cameras operating in parallel allows for the simultaneous acquisition of two channels (e.g. red/green).

Manufacturer product description: UltraView VOX.

Features:

• Available laser lines for excitation (the same are available for FRAP):
  • 405 nm
  • 488 nm
  • 561 nm
  • 640 nm
•  Available emission filters:
  • dual “blue – red” 405 – 475 nm & 580 – 650 nm
  • “green” 500 – 550 nm
  • dual “green – infra-red” 500 – 550 nm & 680 – 750 nm
• Cameras: Two Hamamatsu EM-CCD (ImageEM X2), 512x512pixels, QE~90%.
• Acquisition software: Volocity.
• Stand: Zeiss AxioObserver Z1,  fully automatic.
• Motorized XY stage (ASI MS2000), with a Z piezo, 100 μm range.
• Small environmental chamber with temperature, CO2 and humidity control. Objective heater.
• Zeiss Definite Focus as hardware focus stabilization.

Capabilities:

• Ultra Fast live specimen 5D imaging (XY,C,T). With sub ROI, 90 fps in two colors.
• Confocal imaging.
• Multi-point imaging.
• Stitching of tiled images for large FOV acquisition.
• FRAP, photoactivation, photo-conversion of fluorophores.
• Hardware focus stabilization for long term imaging.

Guide for spinning disk acquisition

Tutorial for spining disk, ultraview acquisition