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© Emmanuel Lemichez
Microscopy image showing the formation of large tunnels in a blood vessel endothelial cell induced by a group of bacterial toxins
Publication : eLife

Dynamic density shaping of photokinetic E. coli

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in eLife - 14 Aug 2018

Frangipane G, Dell'Arciprete D, Petracchini S, Maggi C, Saglimbeni F, Bianchi S, Vizsnyiczai G, Bernardini ML, Di Leonardo R

Link to Pubmed [PMID] – 30103856

Elife 2018 Aug;7

Many motile microorganisms react to environmental light cues with a variety of motility responses guiding cells towards better conditions for survival and growth. The use of spatial light modulators could help to elucidate the mechanisms of photo-movements while, at the same time, providing an efficient strategy to achieve spatial and temporal control of cell concentration. Here we demonstrate that millions of bacteria, genetically modified to swim smoothly with a light controllable speed, can be arranged into complex and reconfigurable density patterns using a digital light projector. We show that a homogeneous sea of freely swimming bacteria can be made to morph between complex shapes. We model non-local effects arising from memory in light response and show how these can be mitigated by a feedback control strategy resulting in the detailed reproduction of grayscale density images.