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© Research
Publication : Acta crystallographica. Section D, Biological crystallography

Ionic strength reducers: an efficient approach to protein purification and crystallization. Application to two Rop variants

Member: Anastasia Gazi
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Acta crystallographica. Section D, Biological crystallography - 22 Jun 2004

Papanikolau Y, Kotsifaki D, Fadouloglou VE, Gazi AD, Glykos NM, Cesareni G, Kokkinidis M

Link to Pubmed [PMID] – 15213408

Acta Crystallogr. D Biol. Crystallogr. 2004 Jul;60(Pt 7):1334-7

Detailed knowledge of the influence of various parameters on macromolecular solubility is essential for crystallization. The concept of so-called ‘ionic strength reducers’ provides insight into the changes in solubility induced by organic solvents and hydrophilic polymers in aqueous electrolytic solutions. A simple and efficient procedure is presented which exploits the properties of ionic strength reducers in the purification and crystallization of proteins. Using two designed variants of the Rop protein as model systems, superior crystals have been obtained compared with conventional techniques. This procedure is particularly useful in cases where excessive nucleation leads to the growth of a large number of tiny crystals that are useless for crystallographic analysis.