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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Publication : Chemical communications (Cambridge, England)

Facile immobilization of DNA using an enzymatic his-tag mimic

Team: Molecular Biophysics
Member: Bruno Baron
Member: Patrick England
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Chemical communications (Cambridge, England) - 05 Dec 2017

Röthlisberger P, Levi-Acobas F, Sarac I, Baron B, England P, Marlière P, Herdewijn P, Hollenstein M

Link to Pubmed [PMID] – 29164188

Chem. Commun. (Camb.) 2017 Dec;53(97):13031-13034

Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.