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© Research
Publication : Archives of microbiology

Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans.

Team: Leptospira adaptation to oxidative stress 
Team: Biology of Spirochetes
Member: Myriam Gominet
Member: Nadia Benaroudj
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Archives of microbiology - 01 Dec 2013

Guyet A, Gominet M, Benaroudj N, Mazodier P

Link to Pubmed [PMID] – 24196782

Link to DOI – 10.1007/s00203-013-0918-2

Arch Microbiol 2013 Dec; 195(12): 831-41

Insertion of an apramycin resistance cassette in the clpP1clpP2 operon (encoding the ClpP1 and ClpP2 peptidase subunits) affects morphological and physiological differentiation of Streptomyces lividans. Another key factor controlling Streptomyces differentiation is the pleiotropic transcriptional regulator AdpA. We have identified a spontaneous missense mutation (-1 frameshift) in the adpA (bldH) open reading frame in a clpP1clpP2 mutant that led to the synthesis of a non-functional AdpA protein. Electrophoretic mobility shift assays showed that AdpA bound directly to clpP1clpP2 promoter region. Quantitative real-time PCR analysis showed that AdpA regulated the clpP1clpP2 operon expression at specific growth times. In vitro, AdpA and ClgR, a transcriptional activator of clpP1clpP2 operon and other genes, were able to bind simultaneously to clpP1 promoter, which suggests that AdpA binding to clpP1 promoter did not affect that of ClgR. This study allowed to uncover an interplay between the ClpP peptidases and AdpA in S. lividans.