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© Institut Pasteur
Cryptococcus neoformans
Publication : Antonie van Leeuwenhoek

Sizing of the Rhodococcus sp. R312 genome by pulsed-field gel electrophoresis. Localization of genes involved in nitrile degradation

Team: RNA Biology of Fungal Pathogens
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Antonie van Leeuwenhoek - 01 Aug 1995

Bigey F, Janbon G, Arnaud A, Galzy P

Link to Pubmed [PMID] – 8546455

Antonie Van Leeuwenhoek 1995 Aug;68(2):173-9

The two restriction enzymes AsnI and DraI were found to produce DNA fragment sizes that could be used for mapping the Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) genome by pulsed-field gel electrophoresis. AsnI produced 24 fragments (4 to 727 kb) and DraI yielded 15 fragments (8.5 to 2400 kb). The fragment lengths in each digest were summed, indicating that the size of the chromosome ranged from 6.31 to 6.56 Mb, with a mean of 6.44 Mb. In addition, the wide-spectrum amidase gene (amiE) and the operon containing the enantiomer-selective amidase gene (amdA) and the nitrile hydratase structural gene (nthA, nthB) were localized on the AsnI and DraI fragments.