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© Research
Publication : FEMS microbiology letters

Characterization of the chromosomal class A beta-lactamase CKO from Citrobacter koseri

Team: Collection of the Institut Pasteur (CIP)
Member: Dominique Clermont
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEMS microbiology letters - 01 Jan 2006

Petrella S, Renard M, Ziental-Gelus N, Clermont D, Jarlier V, Sougakoff W

Link to Pubmed [PMID] – 16445758

FEMS Microbiol. Lett. 2006 Jan;254(2):285-92

The gene bla(CKO) encoding the chromosomal class A beta-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from bla(CKO). Silent and missense mutations were detected in four bla(CKO) genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A bla(CKO)-specific polymerase chain reaction confirmed that bla(CKO) is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.