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Deputy Director of National Reference Center
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Overexpression of proinflammatory TLR-2-signalling lipoproteins in hypervirulent mycobacterial variants

Molecular taxonomy and phylogenetic affinities of two groundwater amphipods, Crangonyx islandicus and Crymostygius thingvallensis, endemic to Iceland

© Institut Pasteur

Phlébotome. Petit diptère de la famille des psychodidés (dans l'Ancien Monde). La femelle hématophage peut être vectrice entre autres de leishmaniose qu'elle transmet par piqûre à un vertébré, chien (hôte principal réservoir), homme ou encore rat (hôtes occasionnels).

Publication : Methods in molecular biology (Clifton, N.J.)

Viral small RNA cloning and sequencing

Team: Viruses and RNA Interference

Member: Carla Saleh

Scientific Fields

Diseases

Organisms

Applications

Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2011

Gausson V, Saleh MC

Link to Pubmed [PMID] – 21431681

Methods Mol. Biol. 2011;721:107-22

At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host-pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).

Published on: 01 Jan 2011