Gataca-Zeiss-Inscoper microscope

This microscope is spinning disk microscope, equipped with a Yokagawa CSU-W1 spinning disk, controlled by the Inscoper software. This system offers different support, suitable for a wide variety of sample types , from subcellular and cellular experiments to large tissue samples and organoids requiring deeper imaging.

Features :

Available light sources

• Transmitted light: brightfield, DIC and phase contrast
• Widefield (excitation with Colibri LED):
  • DAPI
  • GFP
  • CY3
• Confocal (lasers – centre λ/bandwidth) :
  • 405 (445/58 nm)
  • 488 (529/50 nm)
  • 561 (595/44 nm)
  • 638 (706/95 nm)

Available emission filters

• Quad band filter

Objectives

• 5x/0.16: EC Plan-Neofluar, working distance 18.5 mm, Ph 1
• 10x/0.5: Fluar, WD 1.6 mm
• 10x/0.45: Plan Apochromat, WD 2 mm, Ph1
• 20x/0.5: EC Plan-Neofluar, WD 2 mm, Ph2
• 63x/0.75: LD Plan-Neofluar, WD 1.7 mm
• 63x/1.4 : oil immersion Plan Apochromat, WD 0,19 mm

Detectors

• Camera : Prime BSI Express
  • sCMOS,6.5 µm pixel size, 16-12-11 bit, QE 95%field of view: 18.8mm
  • reading speed: until 95 im/s
• Acquisition software : Inscoper 9.2.18 

Capabilities:

• Widefield
• Confocal 
• Fast imaging