About
FAN LIU
Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
External attendees wishing to participate in the seminar are required to contact the following email address dbsc@pasteur.fr in order to obtain access to the Institut Pasteur premises
ABSTRACT
Proteins in all biological systems are highly organized in three-dimensional space, forming membrane-enclosed or membraneless compartments, signaling pathways, dynamic and stable complexes. Many of these structures are only viable in their native environment, making them recalcitrant to traditional biochemical characterization. Proteome-wide cross-linking mass spectrometry offers the opportunity to capture the interactions and spatial arrangement of proteins without having to extract them from their complex biological system.
Over the years, we have advanced cross-linking mass spectrometry through the development of novel experimental methods and computational tools and generated tens of thousands of protein-protein interactions across diverse biological systems, including mitochondria, synapses, cells, and virus particles. These datasets provide insights into protein subcellular localization, interaction networks, and the architecture of supramolecular complexes. Moreover, these discoveries guide functional follow-up studies, facilitating further characterization of novel protein structures, interactions, and spatially resolved networks using techniques from structural biology, molecular biology, cell biology, neuroscience, and bioinformatics.