About
Collaborations: JY Madec (Anses), ENVA, AP-HP, INSERM, CHU Clermont-Ferrand, CNRS, Institut Pasteur
Summary: Intestinal colonization of human individuals with Enterobacterales disseminating resistances to extended-spectrum cephalosporins (ESC-E) and carbapenems (CP-E) is of crucial importance since this phenomenon silently contributes to further ESC-E/CP-E spread within human communities.
DYASPEO aims to conduct a 2-year survey of intestinal carriage of ESC-E/CP-E in 500 dogs and house members (HM) before, during and after dog hospitalization.
It will constitute a unique set of epidemiological data and ESC-E/CP-E isolates, from which we will test several hypotheses on AMR transmission and gut colonization. Hypotheses will be raised on transfer events of ESC-E/CP-E clones and plasmids within and between dogs and HM, on candidate loci underlying host transmission and gut persistence of ESC-E/CP-E, and on within-host evolution pathways of ESC-E/CP-E. Those hypotheses will be tested in cell and in vivo models. Data on impacts of dog hospitalization on dog and human gut metagenomes will be gathered, and dog/human relationships analyzed from a sociological perspective. Finally, all data will be integrated in mathematical models to decipher risk factors of ESC-E/CP-E acquisition by humans from dogs.
DYASPEO will clarify the issue on AMR transmission from companion animals to humans. The consortium of presentes unique complementarities in human and veterinary medicine, clinical and epidemiological studies, molecular genetics, ecology, population genomics and functional approaches, in vitro and in vivo models, computational analyses and social sciences.
Funding: ANR / PPR Antibioresistance
