NanoDSF measures the thermal denaturation of proteins by following the modification of the environment of their fluorescent aminoacids (Tryptophanes and Tyrosines). This label free detection avoids dye interference.
SUPR-DSF (Applied Photophysics) is a plate-based instrument and can perform fast, sensitive, simultaneous analysis of 384 distinct samples using only 10 µL of samples at very low concentrations. The range of experiments is between 10 and 105°C. The software extracts Tm, Tonset and apparent ΔH from intensity and emission scans, enabling rapid stability and ligand‑binding screening.
SUPR-DSF also supports isothermal experiments for applications such as chemical denaturation and affinity measurements.