Lien vers Pubmed [PMID] – 30413354
Lien DOI – 10.1016/j.diagmicrobio.2018.10.008
Diagn Microbiol Infect Dis 2019 Mar; 93(3): 188-190
This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.