Lien vers Pubmed [PMID] – 2088480
Int. Immunol. 1990;2(1):73-81
The dynamic behaviour of isolated populations of peritoneal LPS-reactive (LPSr) B lymphocytes was studied upon transfer of peritoneal cells (PerC) from C57BL/6 LPS responder into C57BL/10ScCr LPS non-responder mice. We have followed the persistence and life-span of the transferred LPSr donor B cells in the spleen and peritoneal cavity of both intact and X-irradiated adult hosts after i.v. or i.p injection and neonatal 1-day-old recipients after i.v. transfer. We have found that lymphocyte life-spans can be influenced by local host environments, as the transferred PerC LPSr cells showed different kinetics according to their route of injection, organ localization, and age or state of the recipients. Thus, while in intact hosts most of the transferred peritoneal LPSr cells decayed with time, following transfer into X-irradiated recipients the same cells were able to expand and replenish the lymphoid tissues of the host. Moreover, upon transfer into intact hosts, the kinetic properties of peritoneal LPSr cells from adult mice differ from splenic LPSr cells of age-matched animals, but mimic those of spleen cells from young, 1-to 2-week-old donors. These findings may reflect the different phenotype composition of adult spleen cells (poor in Ly1 B cells) and peritoneal and neonatal spleen cells (both rich for Ly1 B cells), or may be the result of selective events leading to the peritoneal accumulation of cells with different population dynamics.