Lien DOI – 10.3791/64093
JoVE, 2022 Jul;185:e64093
With a dismal median survival time in real populations-between 6 to 15 monthsglioblastoma
(GBM) is the most devastating malignant brain tumor. Treatment failure
is mainly due to the invasiveness of GBM cells, which speaks for the need for
a better understanding of GBM motile properties. To investigate the molecular
mechanism supporting GBM invasion, new physiological models enabling in-depth
characterization of protein dynamics during invasion are required. These observations
would pave the way to the discovery of novel targets to block tumor infiltration
and improve patient outcomes. This paper reports how an orthotopic xenograft of
GBM cells in the zebrafish brain permits subcellular intravital live imaging. Focusing
on microtubules (MTs), we describe a procedure for MT labeling in GBM cells,
microinjecting GBM cells in the transparent brain of 3 days post fertilization (dpf)
zebrafish larvae, intravital imaging of MTs in the disseminating xenografts, altering MT
dynamics to assess their role during GBM invasion, and analyzing the acquired data.