Lien vers Pubmed [PMID] – 33185870
Lien DOI – 10.1007/978-1-0716-1130-2_7
Methods Mol Biol 2021 ; 2248(): 91-107
Systemic cytokine inhibition may be an effective therapeutic strategy for several autoimmune diseases. However, recent studies suggest that tissue or cell type-specific targeting of certain cytokines, including TNF, may have distinct advantages and show fewer side effects. Here we describe protocols for generating and testing bispecific cytokine inhibitors using variable domain of single-chain antibodies from Camelidae (VHH) with a focus on cell-specific TNF inhibitors.