Expanding substrate specificity of nucleoside 2′-deoxyribosyltransferase

Lien vers Pubmed [PMID] – 18776470

Nucleic Acids Symp Ser (Oxf) 2008;(52):495-6

Nucleoside 2′-deoxyribosyltransferase (NDT) is used to synthesize unnatural 2′-deoxyribonucleosides, modified mostly on the heterocyclic base. Here we describe a strategy for improving 2,3-dideoxyribosyl(ddR) transfer activity of NDT by combining mutagenesis and in vivo selection in E. coli.