Lien vers Pubmed [PMID] – 23463010
Nat Commun 2013;4:1567
Shigella, the agent of bacillary dysentery, invades epithelial cells by locally inducing actin reorganization. Upon cell invasion, Shigella induces calcium (Ca(2+)) signalling, but its role in invasion has remained unclear. Here we show that components involved in inositol 1, 4, 5- trisphosphate (InsP3) signalling are implicated in Shigella invasion. Although global Ca(2+) responses are dispensable for bacterial invasion, local Ca(2+) responses of unprecedented long duration are associated with invasion sites. Fluorescence recovery after photo-bleaching experiments indicate that diffusion of small solutes is hindered at Shigella-invasion sites and that diffusion hindrance is dependent on bacterially induced actin reorganization. Computational simulations and experimental challenge of the model support the notion that local accumulation of InsP3 permitted by restricted diffusion and enrichment of InsP3 receptors account for sustained local Ca(2+) increases at entry sites. Thus, cytoskeletal reorganization through diffusion hindrance shapes the duration of local Ca(2+) signals.