Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient

Lien vers Pubmed [PMID] – 25667013

J. Immunol. Methods 2015 Mar;418:61-5

Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5′ end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5′ end of human Igλ.