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© Research
Publication : Applied and environmental microbiology

Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression.

Team: Group: Catherine Bourgouin
Team: Ecology and Emergence of Arthropod-borne Pathogens
Member: Catherine Bourgouin
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied and environmental microbiology - 01 Feb 1990

Bourgouin C, Delécluse A, de la Torre F, Szulmajster J,

Link to Pubmed [PMID] – 2306087

Appl. Environ. Microbiol. 1990 Feb; 56(2): 340-4

The genes encoding the toxic determinants of Bacillus sphaericus have been expressed in a nontoxic and a toxic strain of Bacillus thuringiensis subsp. israelensis. In both cases, the B. sphaericus toxin proteins were produced at a high level during sporulation of B. thuringiensis and accumulated as crystalline structures. B. thuringiensis transformants expressing B. sphaericus and B. thuringiensis subsp. israelensis toxins did not show a significant enhancement of toxicity against Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae.