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© Research
Publication : Microbiology (Reading, England)

Structure-function relationships of UMP kinases from pyrH mutants of Gram-negative bacteria

Team: Archived: International Group for Data Analysis
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Microbiology (Reading, England) - 01 Jul 2004

Sakamoto H, Landais S, Evrin C, Laurent-Winter C, Bârzu O, Kelln RA

Link to Pubmed [PMID] – 15256558

Microbiology (Reading, Engl.) 2004 Jul;150(Pt 7):2153-9

Bacterial uridine monophosphate (UMP) kinases are essential enzymes encoded by pyrH genes, and conditional-lethal or other pyrH mutants were analysed with respect to structure-function relationships. A set of thermosensitive pyrH mutants from Escherichia coli was generated and studied, along with already described pyrH mutants from Salmonella enterica serovar Typhimurium. It is shown that Arg-11 and Gly-232 are key residues for thermodynamic stability of the enzyme, and that Asp-201 is important for both catalysis and allosteric regulation. A comparison of the amino acid sequence of UMP kinases from several prokaryotes showed that these were conserved residues. Discussion on the enzyme activity level in relation to bacterial viability is also presented.