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© Research
Publication : Antimicrobial agents and chemotherapy

Quinolone resistance in Neisseria gonorrhoeae: rapid genotyping of quinolone resistance-determining regions in gyrA and parC genes by melting curve analysis predicts susceptibility

Member: Frédérique Vernel-Pauillac
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Antimicrobial agents and chemotherapy - 05 Jan 2009

Vernel-Pauillac F, Hogan TR, Tapsall JW, Goarant C

Link to Pubmed [PMID] – 19124663

Antimicrob. Agents Chemother. 2009 Mar;53(3):1264-7

We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.