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© Research
Publication : Science (New York, N.Y.)

Nucleotide sequence and expression of the diphtheria tox228 gene in Escherichia coli

Team: Archived: Biology of Enteric Viruses
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Science (New York, N.Y.) - 26 Aug 1983

Kaczorek M, Delpeyroux F, Chenciner N, Streeck RE, Murphy JR, Boquet P, Tiollais P

Link to Pubmed [PMID] – 6348945

Science 1983 Aug;221(4613):855-8

The complete nucleotide sequence of the diphtheria tox228 gene encoding the nontoxic serologically related protein CRM228 has been determined. A comparison of the predicted amino acid sequence with the available amino acid sequences from the wild-type toxin made it possible to deduce essentially the entire nucleotide sequence of the wild-type tox gene. The signal peptide of pro-diphtheria toxin and the putative tox promoter have been identified, a highly symmetrical nucleotide sequence downstream of the toxin gene has been detected; this region may be the corynebacteriophage beta attachment site (attP). The cloned toxin gene was expressed at a low level in Escherichia coli.