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© Research
Publication : Acta biochimica Polonica

New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures

Team: Archived: Experimental neuropathology
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Acta biochimica Polonica - 11 Jan 2010

El Tahchy A, Boisbrun M, Ptak A, Dupire F, Chrétien F, Henry M, Chapleur Y, Laurain-Mattar D

Link to Pubmed [PMID] – 20066175

Acta Biochim. Pol. 2010;57(1):75-82

Biotransformation of deuterated-4′-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4′-O-methyl-d(3)-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.