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© Research
Publication : Nucleic acids research

Molecular cloning and nucleotide sequence of a human renin cDNA fragment

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 25 Oct 1983

Soubrier F, Panthier JJ, Corvol P, Rougeon F

Link to Pubmed [PMID] – 6138751

Nucleic Acids Res. 1983 Oct;11(20):7181-90

We have studied human renin messenger RNA by hybridization with the mouse submaxillary gland (SMG) renin cDNA probe. The human kidney messenger RNA is about 1.6 kilobase (kb) long, similarly to the mouse SMG renin mRNA. A kidney renin cDNA clone of 1.1 kb length was obtained. A comparison of nucleotide sequences of mouse and human cDNA clones reveals conservation of residues involved in catalytic mechanisms and a potential glycosylation site. The human renin molecular probe allowed us to study renin expression in human chorionic tissue. The chorionic and kidney renin messenger RNAs are similar in length. The Southern blot analysis reveals the presence of a single renin gene in human DNA.