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© Research
Publication : The Journal of experimental medicine

Localization of gamma/delta T cells to the intestinal epithelium is independent of normal microbial colonization

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of experimental medicine - 01 Jul 1990

Bandeira A, Mota-Santos T, Itohara S, Degermann S, Heusser C, Tonegawa S, Coutinho A

Link to Pubmed [PMID] – 2141628

J. Exp. Med. 1990 Jul;172(1):239-44

Using monoclonal antibodies identifying all gamma/delta and alpha/beta T cell receptors in cytofluorometric analysis, we have compared the composition of intestinal intraepithelial lymphocytes (i-IEL) in euthymic and athymic germ-free (GF) and conventional (SPF) mice. The results show a marked influence of microbial colonization in the numbers of single-positive (CD4+ or CD8+) alpha/beta i-IEL, but little effect in the pool size or characteristics of gamma/delta i-IEL. In young athymic mice, virtually no alpha/beta i-IEL are detected, while considerable numbers of gamma/delta i-IEL remain, though reduced in GF animals.