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© Research
Publication : Applied and environmental microbiology

Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei

Member: Philippe Sansonetti
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied and environmental microbiology - 18 May 2012

Licandro-Seraut H, Brinster S, van de Guchte M, Scornec H, Maguin E, Sansonetti P, Cavin JF, Serror P

Link to Pubmed [PMID] – 22610425

Appl. Environ. Microbiol. 2012 Aug;78(15):5417-23

The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.