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© Alexandre Alanio
Cryptocccus neoformans cells collected from lung of infected mice and stained with calcofluor (blue) and with anti-capsule antibody (green).
Publication : The Lancet Microbe

Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study

Team: Translational Mycology
Reference Center: Invasive Mycoses and Antifungals
Network: Institut Pasteur
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Lancet Microbe - 08 Feb 2024

Tshepiso Mbangiwa, PhD Aude Sturny-Leclère, MSc Kwana Lechiile, MSc Cheusisime Kajanga, MSc Timothée Boyer-Chammard, MD Jennifer C Hoving, PhD

Link to DOI – 10.1016/S2666-5247(23)00362-2

Background

HIV-associated cryptococcal meningitis is the second leading cause of AIDS-related deaths, with a 10-week mortality rate of 25–30%. Fungal load assessed by colony-forming unit (CFU) counts is used as a prognostic marker and to monitor response to treatment in research studies. PCR-based assessment of fungal load could be quicker and less labour-intensive. We sought to design, optimise, and validate quantitative PCR (qPCR) assays for the detection, identification, and quantification of Cryptococcus infections in patients with cryptococcal meningitis in sub-Saharan Africa.