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© Research
Publication : Journal of clinical microbiology

Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR

Team: Archived: Molecular Genetics of RNA Viruses
Member: Bernadette Crescenzo
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of clinical microbiology - 01 Jun 1996

Turkoglu S, Lazizi Y, Meng H, Kordosi A, Dubreuil P, Crescenzo B, Benjelloun S, Nordmann P, Pillot J

Link to Pubmed [PMID] – 8735122

J. Clin. Microbiol. 1996 Jun;34(6):1568-71

Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.