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  • Director of Center
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© Research
Publication : The American journal of tropical medicine and hygiene

Dengue-3 outbreak in Paraguay: investigations using capillary blood samples on filter paper.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The American journal of tropical medicine and hygiene - 01 Nov 2008

Matheus S, Meynard JB, Lavergne A, Girod R, Moua D, Labeau B, Dussart P, Lacoste V, Deparis X

Link to Pubmed [PMID] – 18981504

Am J Trop Med Hyg 2008 Nov; 79(5): 685-7

During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the techniques used. Molecular analyses detected the dengue-3 serotype in 22 patients and additionally the dengue-2 serotype in two patients. Therefore two different serotypes were co-circulating during this outbreak. Overall, this study validates the use of dried-blood samples for field screening investigations. Indeed, all types of laboratory studies of dengue were possible with samples consisting of a few drops of dried blood from finger pricks.