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  • Director of Center
  • Director of Department
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© Research
Publication : Methods in enzymology

Correlated light and electron cryo-microscopy.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in enzymology - 01 Jan 2010

Briegel A, Chen S, Koster AJ, Plitzko JM, Schwartz CL, Jensen GJ

Link to Pubmed [PMID] – 20887863

Link to DOI – 10.1016/S0076-6879(10)81013-4

Methods Enzymol 2010 ; 481(): 317-41

Light and electron cryo-microscopy have each proven to be powerful tools to study biological structures in a near-native state. Light microscopy provides important localization information, while electron microscopy provides the resolution necessary to resolve fine structural details. Imaging the same sample by both light and electron cryo-microscopy is a powerful new approach that combines the strengths of both techniques to provide novel insights into cellular ultrastructure. In this chapter, the methods and instrumentation currently used to correlate light and electron cryo-microscopy are described in detail.