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Publication : Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis

Complete mitochondrial genome of Lutzomyia (Nyssomyia) umbratilis (Diptera: Psychodidae), the main vector of Leishmania guyanensis.

Member: Romain Girod
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis - 01 Nov 2016

Kocher A, Gantier JC, Holota H, Jeziorski C, Coissac E, Bañuls AL, Girod R, Gaborit P, Murienne J

Link to Pubmed [PMID] – 26000939

Mitochondrial DNA A DNA Mapp Seq Anal 2016 Nov; 27(6): 4219-4221

The nearly complete mitochondrial genome of Lutzomyia umbratilis Ward & Fraiha, 1977 (Psychodidae: Phlebotominae), considered as the main vector of Leishmania guyanensis, is presented. The sequencing has been performed on an Illumina Hiseq 2500 platform, with a genome skimming strategy. The full nuclear ribosomal RNA segment was also assembled. The mitogenome of L. umbratilis was determined to be at least 15,717 bp-long and presents an architecture found in many mitogenomes of insect (13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and one non-coding region also referred as the control region). The control region contains a large repeated element of c. 370 bp and a poly-AT region of unknown length. This is the first mitogenome of Psychodidae to be described.