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© Research
Publication : Proceedings of the National Academy of Sciences of the United States of America

cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proceedings of the National Academy of Sciences of the United States of America - 01 Apr 1982

Robert B, Weydert A, Caravatti M, Minty A, Cohen A, Daubas P, Gros F, Buckingham M

Link to Pubmed [PMID] – 6283523

Proc Natl Acad Sci U S A 1982 Apr; 79(8): 2437-41

A recombinant plasmid with a cDNA sequence transcribed from mouse skeletal muscle RNA is shown to hybridize with mRNAs for myosin light chains LC1F and LC3F. The inserted fragment corresponds exclusively to the 3′-noncoding region of the mRNA. It hybridizes almost exclusively with the two light chain messengers from fast skeletal muscle RNA of adult mouse. Slight hybridization is seen with RNA from heart muscle and embryonic skeletal muscle. The implications of the conservation of the 3′-noncoding regions between the two mRNAs are discussed.