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© Research
Publication : Gene

An IS15 insertion generates an eight-base-pair duplication of the target DNA

Team: Archived: Group : Patrick Trieu-Cuot
Member: Patrick Trieu-Cuot
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Gene - 01 Sep 1983

Trieu-Cuot P, Labigne-Roussel A, Courvalin P

Link to Pubmed [PMID] – 6313479

Gene 1983 Sep;24(1):125-9

In plasmid pIP1088 the transposable module IS15 is inserted at nucleotide position 1,430 of the vector plasmid pBR322. We have sequenced the termini of the IS15 element, which consists of two perfect inverted repeat sequences, 14 bp long. The sequence is 5′-GGCACTGTTGCAAA . . . TTTGCAACAGTGCC-3′. The integration event results in the duplication of 8 bp of target DNA.