This one-week course presents a combination of lectures and intensive hands-on sessions to learn the most up-to-date CARD (*Center For Animal Resources and Development) methods in mouse cryopreservation.
Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have pushed the envelope of mouse cryopreservation: i) improved female superovulation method; ii) fresh, frozen and cold storage sperm techniques; iii) optimized IVF methods. These improvements (see main references in program) have resulted in an unparalleled increase in efficiency of cryopreservation and rescue of relevant mouse lines.
The aim of this course is to introduce the newest CARD methods to researchers and technicians involved in mouse archiving and/or managing transgenic facilities and who are willing to implement these new methods in their work. These techniques will be taught directly by the team that devised them.
Each participant will have one binocular microscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field.
– See more at: http://www.pasteur.fr/fr/enseignement/ateliers/mouse-sperm-and-embryo-cryopreservation-course#sthash.uu1hgxfG.dpuf
Location
Location: 25-28 Rue du Dr Roux, 75015 Paris, France
(function($) {
/*
* render_map
*
* This function will render a Google Map onto the selected jQuery element
*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
* @param $el (jQuery element)
* @return n/a
*/
function render_map( $el ) {
var MY_MAPTYPE_ID = 'custom_style';
var featureOpts = [{"stylers":[{"hue":"#0088ff"}]}];
var styledMapOptions = {
name: 'Custom Style'
};
var customMapType = new google.maps.StyledMapType(featureOpts, styledMapOptions);
// var
var $markers = $el.find('.marker');
// vars
var args = {
zoom : 16,
scrollwheel : false,
center : new google.maps.LatLng(0, 0),
mapTypeId : google.maps.MapTypeId.ROADMAP,
mapTypeId : MY_MAPTYPE_ID
};
// create map
var map = new google.maps.Map( $el[0], args);
map.mapTypes.set(MY_MAPTYPE_ID, customMapType);
// add a markers reference
map.markers = [];
// add markers
$markers.each(function(){
add_marker( $(this), map );
});
// center map
center_map( map );
}
/*
* add_marker
*
* This function will add a marker to the selected Google Map
*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
* @param $marker (jQuery element)
* @param map (Google Map object)
* @return n/a
*/
function add_marker( $marker, map ) {
// var
var latlng = new google.maps.LatLng( $marker.attr('data-lat'), $marker.attr('data-lng') );
//var image = 'https://research.pasteur.fr/wp-content/themes/biologyx2/images/logomap.png';
var myIcon = new google.maps.MarkerImage('https://research.pasteur.fr/wp-content/themes/biologyx2/images/map-pin.png', null, null, null, new google.maps.Size(32,32));
// create marker
var marker = new google.maps.Marker({
position : latlng,
map : map,
icon : myIcon
});
// add to array
map.markers.push( marker );
// if marker contains HTML, add it to an infoWindow
if( $marker.html() )
{
// create info window
var infowindow = new google.maps.InfoWindow({
content : $marker.html()
});
// show info window when marker is clicked
google.maps.event.addListener(marker, 'click', function() {
infowindow.open( map, marker );
});
}
}
/*
* center_map
*
* This function will center the map, showing all markers attached to this map
*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
* @param map (Google Map object)
* @return n/a
*/
function center_map( map ) {
// vars
var bounds = new google.maps.LatLngBounds();
// loop through all markers and create bounds
$.each( map.markers, function( i, marker ){
var latlng = new google.maps.LatLng( marker.position.lat(), marker.position.lng() );
bounds.extend( latlng );
});
// only 1 marker?
if( map.markers.length == 1 )
{
// set center of map
map.setCenter( bounds.getCenter() );
map.setZoom( 15 );
}
else
{
// fit to bounds
map.fitBounds( bounds );
}
}
/*
* document ready
*
* This function will render each map when the document is ready (page has loaded)
*
* @type function
* @date 8/11/2013
* @since 5.0.0
*
* @param n/a
* @return n/a
*/
$(document).ready(function(){
$('.acf-map').each(function(){
render_map( $(this) );
});
});
})(jQuery);
