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© Research
Publication : Ticks and tick-borne diseases

Of fungi and ticks: Morphological and molecular characterization of fungal contaminants of a laboratory-reared Ixodes ricinus colony.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Ticks and tick-borne diseases - 01 Sep 2021

Bonnet SI, Blisnick T, Al Khoury C, Guillot J

Link to Pubmed [PMID] – 33992909

Link to HAL – hal-03269146

Link to DOI – 10.1016/j.ttbdis.2021.101732

Ticks Tick Borne Dis 2021 Sep; 12(5): 101732

Establishing and maintaining tick colonies in the laboratory is essential for studying their biology and pathogen transmission, or for the development of new tick control methods. Due to their requirement for very high humidity, these laboratory-bred colonies are frequently subject to fungal contamination. In the present study, we aimed to identify the fungal species that contaminated a laboratory-reared colony of Ixodes ricinus through microscopic observation and molecular identification. We identified three different taxa isolated from the ticks: Aspergillus parasiticus, Penicillium steckii, and Scopulariopsis brevicaulis. These three species are usually regarded as environmental saprophytic molds but both direct and indirect evidence suggest that they could also be considered as entomopathogenic fungi. Although we do not have any direct evidence that the fungi isolated from I. ricinus in this study could cause lethal infections in ticks, we observed that once infected, heavy fungal growth coupled with very high mortality rates suggest that studying the entomopathogenic potential of these fungi could be relevant to biological tick control.