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© Research
Publication : Advances in experimental medicine and biology

Production of influenza virus in cell cultures for vaccine preparation

Member: Stéphane Petres
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Advances in experimental medicine and biology - 01 Jan 1996

Merten OW, Hannoun C, Manuguerra JC, Ventre F, Petres S

Link to Pubmed [PMID] – 8718593

Adv. Exp. Med. Biol. 1996;397:141-51

Influenza virus strains of different types for use as an inactivated vaccine have been successfully grown in different cell lines. Increasing titres were obtained with BHK-21/BRS, VERO and MDCK cells. Cultures in stationary flasks, in spinner cultures or in large bioreactor systems were tested and the optimal conditions were studied. MDCK cells grown in serum-free medium before and during the virus production phase were found to yield high titres in the presence of trypsin. Satisfactory results were obtained with egg-adapted strains of human and equine origin as well as with strains just isolated from human patients without any further passages in eggs or cell culture.