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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Protocol for Generating Infectious RNA Viromes from Complex Biological Samples.

Team: Pathogen discovery
Member: Sarah Temmam
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2018

Monteil-Bouchard S, Temmam S, Desnues C,

Link to Pubmed [PMID] – 30128987

Link to DOI – 10.1007/978-1-4939-8682-8_3

Methods Mol Biol 2018 ; 1838(): 25-36

This chapter proposes a simple, standardized protocol for generating RNA viromes from complex host-associated biological samples of various origins. Compared to other existing protocols to generate RNA viromes, this protocol preserves the infectivity of viral particles and allows for downstream applications such as viral characterization and isolation tests.