Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search

← Go to Research

Go back
Scroll to top
Share
© Perrine Bomme, Guillaume Duménil, Jean-Marc Panaud.
Coloured scanning electron micrograph (SEM) of Neisseria meningitidis on epithelial cells
Publication : Research in microbiology

RegF, an SspA homologue, regulates the expression of the Neisseria gonorrhoeae pilE gene

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Research in microbiology - 01 May 1997

De Reuse H, Taha MK

Link to Pubmed [PMID] – 9765808

Res. Microbiol. 1997 May;148(4):289-303

The Neisseria gonorrhoeae pilE gene codes for a type IV pilin, the major subunit of pili which constitute an essential virulence factor during gonococcal infection. Expression of pilE seems to be highly regulated, which may allow piliation to adapt to growth conditions. From an N. gonorrhoeae genomic library, we selected plasmid pNG200 encoding a protein (RegF) which caused a 5-fold increase in the expression of pilE::cat fusion in Escherichia coli. This regulation was mediated via the complex pilE promoter region, comprising potential sigma 70- and sigma 54-dependent promoters, and could not be observed in the absence of an active sigma 54 factor. The RegF protein (23,149 Da) showed 42% identity with the E. coli “stringent starvation protein”, SspA. This protein was shown to interact with the RNA polymerase holoenzyme and to play a role in the expression of at least 11 proteins in E. coli. In an N. gonorrhoeae strain carrying a regF::mTn3Cm3 mutation constructed by allelic exchange, it was observed that pilin expression was enhanced. Our results were consistent with a model in which (i) in N. gonorrhoeae, RegF acts as a negative regulator of pilE transcription, and (ii) in E. coli, RegF increases pilE transcription by preventing sigma 54-associated steric hindrance at pilE promoters described previously.