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© Research
Publication : The Journal of biological chemistry

The nucleotide sequences of the two glutamine transfer ribonucleic acids from Escherichia coli

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of biological chemistry - 10 May 1975

Yaniv M, Folk WR

Link to Pubmed [PMID] – 164464

J. Biol. Chem. 1975 May;250(9):3243-53

The nucleotide sequences of the two glutamine tRNA species in Escherichia coli K12 have been determined. Sufficient data was obtained to order unambiguously the products of complete RNase digestion of tRNA2Gln, and all but one oligonucleotide from tRNA1Gln. The sequence of tRNA1Gln was established by analogy with tRNA1Gln, as the two tRNAs are very similar, differing by only 7 residues out of 75. tRNA1Gln has the anticodon NUG, where N is a modified nucleotide which is likely to be a derivative of 2-thiouridine, and is specific for the codon CAA. tRNA1Gln has the anticodon CUG, and is specific for the codon CAG (Folk, W. R., and Yaniv, M. (1972) Nature 237, 165). The complete sequences of the tRNAGln species are: See journal for formula (Unique residues are enclosed in parentheses, with the residue in tRNA1Gln above that in tRNA2Gln.).