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© Charles Dauguet
Virus VIH-1 (HIV-1), agent du sida, à la surface d'un lymphocyte. Image colorisée.
Publication : Science (New York, N.Y.)

Adaptation of lymphadenopathy associated virus (LAV) to replication in EBV-transformed B lymphoblastoid cell lines

Team: Group: MISTIC
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Science (New York, N.Y.) - 01 Jul 1984

Montagnier L, Gruest J, Chamaret S, Dauguet C, Axler C, Guétard D, Nugeyre MT, Barré-Sinoussi F, Chermann JC, Brunet JB

Link to Pubmed [PMID] – 6328661

Science 1984 Jul;225(4657):63-6

A strain of lymphadenopathy associated retrovirus ( LAV ) passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with Epstein-Barr virus of B lymphocytes from a healthy donor. The virus produced from this line (B- LAV ) was also able to grow at a high rate in some other lymphoblastoid lines and in a Burkitt lymphoma line. This adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain, as well as its tropism for normal T4+ lymphocytes. It is thus possible to grow LAV in large quantities that can be used for the preparation of diagnostic reagents. The interaction between such a human retrovirus and Epstein-Barr virus, a DNA virus, may have some implication for the pathology of the acquired immunodeficiency syndrome and related diseases.