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© Research
Publication : Optics express

Fast STED microscopy with continuous wave fiber lasers

Member: Gaël Moneron
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Optics express - 18 Jan 2010

Moneron G, Medda R, Hein B, Giske A, Westphal V, Hell SW

Link to Pubmed [PMID] – 20173956

Opt Express 2010 Jan;18(2):1302-9

We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy in the visible range using for resolution enhancement compact, low cost and turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial resolutions of 35 to 65 nm in the focal plane are shown for various samples including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive selection of 5 commonly used organic fluorescent markers, and living cells expressing the yellow fluorescent protein Citrine. The potential of the straightforward combination of CW-STED and fast beam scanning is illustrated in a movie of the endoplasmic reticulum (ER) of a living cell, composed of 100 frames (6 microm x 12 microm), each of them acquired in a time shorter than 0.2 s.