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Characterisation of early mucosal and neuronal lesions following Shigella flexneri infection in human colon

The regulatory mechanisms that underlie inappropriate transcription of the myogenic determination gene Myf5 in the central nervous system.

© Research

Publication : Journal of clinical microbiology

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

Team: Archived: Molecular Mycology

Member: Eveline Snelders

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Diseases

Organisms

Applications

Technique

Published in Journal of clinical microbiology - 04 Mar 2009

Hurst SF, Kidd SE, Morrissey CO, Snelders E, Melchers WJ, Castelli MV, Mellado E, Simmon K, Petti CA, Richardson S, Zhang S, Romanelli AM, Wickes BL, de Valk HA, Klaassen CH, Balajee SA

Link to Pubmed [PMID] – 19261791

J. Clin. Microbiol. 2009 May;47(5):1562-4

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Published on: 04 Mar 2009