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© Research
Publication : Antimicrobial agents and chemotherapy

Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae

Member: Frédérique Vernel-Pauillac
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Antimicrobial agents and chemotherapy - 25 Aug 2008

Page S, Vernel-Pauillac F, O'Connor O, Bremont S, Charavay F, Courvalin P, Goarant C, Le Hello S

Link to Pubmed [PMID] – 18725440

Antimicrob. Agents Chemother. 2008 Nov;52(11):4155-8

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.