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© Research
Publication : Proteomics

MALDI-TOF MS-based drug susceptibility testing of pathogens: the example of Candida albicans and fluconazole

Team: Archived: Molecular Mycology
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proteomics - 01 Oct 2009

Marinach C, Alanio A, Palous M, Kwasek S, Fekkar A, Brossas JY, Brun S, Snounou G, Hennequin C, Sanglard D, Datry A, Golmard JL, Mazier D

Link to Pubmed [PMID] – 19750514

Proteomics 2009 Oct;9(20):4627-31

MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute’s reference method. This proof-of-concept demonstration highlights the potential for this approach to test other pathogens.