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© Research
Publication : Journal of virological methods

A simple and sensitive method for determining the strand orientation of single-stranded viral genomes

Team: Archived: Viruses of Hyperthermophilic Archaea
Team: Archived: Molecular Biology of Gene in Extremophiles
Member: David Prangishvili
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virological methods - 29 May 2012

Mochizuki T, Prangishvili D

Link to Pubmed [PMID] – 22659067

J. Virol. Methods 2012 Oct;185(1):149-51

Determining the nature of the viral genome is one of the first steps in characterization of any new virus. However, in the case of viruses with a single-stranded genome, it is not always simple to identify its orientation. In this study, a rapid, sensitive and simple PCR-based method is described to identify the strand orientation of single-stranded viral genomes. This method has been tested on the single-stranded DNA viruses, M13 and phiX174.