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© Perrine Bomme, Guillaume Duménil, Jean-Marc Panaud.
Coloured scanning electron micrograph (SEM) of Neisseria meningitidis on epithelial cells
Publication : Clinical laboratory

Proficiency of PCR in hospital settings for nonculture diagnosis of invasive meningococcal infections.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Clinical laboratory - 01 Jan 2012

Hong E, Barraud O, Bidet P, Bingen E, Blondiaux N, Bonacorsi S, Burucoa C, Carrer A, Fortineau N, Couetdic G, Courcol R, Garnier F, Hery-Arnaud G, Lanotte P, Le Bars H, Legrand-Quillien MC, Lemée L, Mereghetti L, Millardet C, Minet J, Plouzeau-Jayle C, Pons JL, Schneider J, Taha MK,

Link to Pubmed [PMID] – 22582511

Clin Lab 2012 ; 58(3-4): 343-6

Meningococcal meningitis requires rapid diagnosis and immediate management which is enhanced by the use of PCR for the ascertainment of these infections. However, its use is still restricted to reference laboratories.We conducted an inter-laboratory study to assess the implementation and the performance of PCR in ten French hospital settings in 2010.Our data are in favour of this implementation. Although good performance was obtained in identifying Neisseria meningitidis positive samples, the main issue was reported in identifying other species (Streptococcus pneumoniae and Haemophilus influenzae) which are also involved in bacterial meningitis cases.Several recommendations are required and, mainly, PCR should target the major etiological agents (N. meningitidis, S. pneumonia, and H. influenzae) of acute bacterial meningitis. Moreover, PCR should predict the most frequent serogroups of Neisseria meningitidis according to local epidemiology.