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© Research
Publication : Applied optics

Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source.

Team: Dynamics of Biological Identities
Member: Jack Christophe Cossec
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied optics - 20 Jan 2009

Blandin P, Lévêque-Fort S, Lécart S, Cossec JC, Potier MC, Lenkei Z, Druon F, Georges P

Link to Pubmed [PMID] – 19151824

Appl Opt 2009 Jan; 48(3): 553-9

We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.