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  • nrc
  • whocc
  • project
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  • tool
  • patent
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  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • MD-PhD Student
  • Medical Staff
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
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  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
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  • Head of Operations
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© Research
Equipment

Focused Ion Beam Scanning Electron Microscope

Scientific Fields
Diseases
Organisms
Applications
Technique

Zeiss • Crossbeam 350

FIB-SEM cross beam, latest generation Carl Zeiss installed in 2025. FIB-SEM (Focused Ion Beam – Scanning Electron Microscopy) is a technique widely used in volumeEM (Electron Microscopy) to reconstruct high-resolution biological structures in 3D. The focused ion beam (gallium) is used to mill (remove very thin layers from the sample block, 3 to 10 nm thick). After each ablation, the SEM takes an image of the newly exposed surface. The sequence of images forms a stack of serial sections that can be reconstructed in 3D. FIB-SEM is now one of the benchmark techniques for EM volume imaging because it combines high-resolution isotropic imaging, automation, and accurate 3D reconstruction of cellular or tissue volumes.
This FIB-SEM is equipped with a Sculptor column, two backscattered electron detectors, and secondary electron detectors.

Zeiss • Auriga

 

Focused ion beam scanning EM is typically applied to analyze whole cells in 3D. In 2016 Atlas-5 was installed on our Auriga with the goal to establish the automated imaging of serial sections, which finds its application in the analysis of the 3D organization of tissue. During the course of 2017 the Auriga will be equipped with a cryo-transfer station enabling for the 3D analysis of samples preserved by freezing only.