Virus interactomics

As a major pathway of reversible protein modification in eukaryotes cells, leading to their degradation, affecting their subcellular localization, or activity, the Ubiquitin Proteasome System (UPS) is a prime target of viruses. Recent evidence […]

Despite medical progress, the recent outbreaks of viral zoonoses (e.g. MERS-CoV, Ebola virus disease) illustrate the constant threat represented by viruses in a world in motion and globalization. These epidemic events stress the need […]

To date, knowledge on the interaction network between filovirus proteins and the host proteome (virhostome) are still extremely small and fragmented. These studies are limited to a few proteins such as VP24, VP35 and […]

The use of light from the abyss in biology   In the last decades, bioluminescent systems based on the expression of a luciferase and the addition of a luciferin to monitor the emission of […]

we developed an optogenetic “Cre-stiching” strategy for barcode fusion genetics. This new technology is based on light-inducible Cre-recombinase complementation. The resulting optogenetic control Cre-recombinase produces a concatenated DNA, linking together genetic barcodes. These concatenated […]

The coverage and robustness of protein-protein interactions (PPI) datasets can be markedly increased by combining orthogonal PPI assays. For this purpose we have recently developed two variations of our High-throughput Gaussia princeps Complementation Assay(HT-GPCA), […]